RapamycinFungusV1, Main, Exploration, bibRecord, 000C08

TORC1 inhibition induces lipid droplet replenishment in yeast.

Identifieur interne : 000C08 ( Main/Exploration ); précédent : 000C07; suivant : 000C09

TORC1 inhibition induces lipid droplet replenishment in yeast.

Auteurs : Juliana B. Madeira [Brésil] ; Claudio A. Masuda [Brésil] ; Clarissa M. Maya-Monteiro [Brésil] ; Gabriel Soares Matos [Brésil] ; M Nica Montero-Lomelí [Brésil] ; Bruno L. Bozaquel-Morais [Brésil]

Source :

Molecular and cellular biology [ 1098-5549 ] ; 2015.

RBID : pubmed:25512609

Descripteurs français

English descriptors

KwdEn :
- Adaptor Proteins, Signal Transducing (genetics), Adaptor Proteins, Signal Transducing (metabolism), Basic Helix-Loop-Helix Leucine Zipper Transcription Factors (genetics), Basic Helix-Loop-Helix Leucine Zipper Transcription Factors (metabolism), Cholesterol Esters (metabolism), GATA Transcription Factors (deficiency), GATA Transcription Factors (genetics), Gene Expression Regulation, Fungal (MeSH), Lipid Droplets (chemistry), Lipid Droplets (drug effects), Lipid Droplets (metabolism), Lipid Metabolism (drug effects), Osmotic Pressure (MeSH), Phosphatidylinositol 3-Kinases (genetics), Phosphatidylinositol 3-Kinases (metabolism), Phosphoinositide-3 Kinase Inhibitors (MeSH), Protein Phosphatase 2 (genetics), Protein Phosphatase 2 (metabolism), Saccharomyces cerevisiae (drug effects), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae Proteins (antagonists & inhibitors), Saccharomyces cerevisiae Proteins (genetics), Saccharomyces cerevisiae Proteins (metabolism), Signal Transduction (MeSH), Sirolimus (pharmacology), Sorbitol (pharmacology), Transcription Factors (antagonists & inhibitors), Transcription Factors (deficiency), Transcription Factors (genetics), Transcription Factors (metabolism), Triglycerides (biosynthesis).
MESH :
- chemical , antagonists & inhibitors : Saccharomyces cerevisiae Proteins, Transcription Factors.
- chemical , biosynthesis : Triglycerides.
- chemical , deficiency : GATA Transcription Factors, Transcription Factors.
- chemical , genetics : Adaptor Proteins, Signal Transducing, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, GATA Transcription Factors, Protein Phosphatase 2, Saccharomyces cerevisiae Proteins, Transcription Factors.
- chemical , metabolism : Adaptor Proteins, Signal Transducing, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Cholesterol Esters, Protein Phosphatase 2, Saccharomyces cerevisiae Proteins, Transcription Factors.
- chemistry : Lipid Droplets.
- drug effects : Lipid Droplets, Lipid Metabolism, Saccharomyces cerevisiae.
- genetics : Phosphatidylinositol 3-Kinases, Saccharomyces cerevisiae.
- metabolism : Lipid Droplets, Phosphatidylinositol 3-Kinases, Saccharomyces cerevisiae.
- chemical , pharmacology : Sirolimus, Sorbitol.
- Gene Expression Regulation, Fungal, Osmotic Pressure, Phosphoinositide-3 Kinase Inhibitors, Signal Transduction.

Abstract

Lipid droplets (LDs) are intracellular structures that regulate neutral lipid homeostasis. In mammals, LD synthesis is inhibited by rapamycin, a known inhibitor of the mTORC1 pathway. In Saccharomyces cerevisiae, LD dynamics are modulated by the growth phase; however, the regulatory pathways involved are unknown. Therefore, we decided to study the role of the TORC1 pathway on LD metabolism in S. cerevisiae. Interestingly, rapamycin treatment resulted in a fast LD replenishment and growth inhibition. The discovery that osmotic stress (1 M sorbitol) also induced LD synthesis but not growth inhibition suggested that the induction of LDs in yeast is not a secondary response to reduced growth. The induction of LDs by rapamycin was due to increased triacylglycerol but not sterol ester synthesis. Induction was dependent on the TOR downstream effectors, the PP2A-related phosphatase Sit4p and the regulatory protein Tap42p. The TORC1-controlled transcriptional activators Gln3p, Gat1p, Rtg1p, and Rtg3p, but not Msn2p and Msn4p, were required for full induction of LDs by rapamycin. Furthermore, we show that the deletion of Gln3p and Gat1p transcription factors, which are activated in response to nitrogen availability, led to abnormal LD dynamics. These results reveal that the TORC1 pathway is involved in neutral lipid homeostasis in yeast.

DOI: 10.1128/MCB.01314-14
PubMed: 25512609
PubMed Central: PMC4301715

Affiliations:

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptor Proteins, Signal Transducing (genetics)</term>
<term>Adaptor Proteins, Signal Transducing (metabolism)</term>
<term>Basic Helix-Loop-Helix Leucine Zipper Transcription Factors (genetics)</term>
<term>Basic Helix-Loop-Helix Leucine Zipper Transcription Factors (metabolism)</term>
<term>Cholesterol Esters (metabolism)</term>
<term>GATA Transcription Factors (deficiency)</term>
<term>GATA Transcription Factors (genetics)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Lipid Droplets (chemistry)</term>
<term>Lipid Droplets (drug effects)</term>
<term>Lipid Droplets (metabolism)</term>
<term>Lipid Metabolism (drug effects)</term>
<term>Osmotic Pressure (MeSH)</term>
<term>Phosphatidylinositol 3-Kinases (genetics)</term>
<term>Phosphatidylinositol 3-Kinases (metabolism)</term>
<term>Phosphoinositide-3 Kinase Inhibitors (MeSH)</term>
<term>Protein Phosphatase 2 (genetics)</term>
<term>Protein Phosphatase 2 (metabolism)</term>
<term>Saccharomyces cerevisiae (drug effects)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Saccharomyces cerevisiae Proteins (antagonists & inhibitors)</term>
<term>Saccharomyces cerevisiae Proteins (genetics)</term>
<term>Saccharomyces cerevisiae Proteins (metabolism)</term>
<term>Signal Transduction (MeSH)</term>
<term>Sirolimus (pharmacology)</term>
<term>Sorbitol (pharmacology)</term>
<term>Transcription Factors (antagonists & inhibitors)</term>
<term>Transcription Factors (deficiency)</term>
<term>Transcription Factors (genetics)</term>
<term>Transcription Factors (metabolism)</term>
<term>Triglycerides (biosynthesis)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Cholestérol ester (métabolisme)</term>
<term>Facteurs de transcription (antagonistes et inhibiteurs)</term>
<term>Facteurs de transcription (déficit)</term>
<term>Facteurs de transcription (génétique)</term>
<term>Facteurs de transcription (métabolisme)</term>
<term>Facteurs de transcription GATA (déficit)</term>
<term>Facteurs de transcription GATA (génétique)</term>
<term>Facteurs de transcription à motifs basiques hélice-boucle-hélice et à glissière à leucines (génétique)</term>
<term>Facteurs de transcription à motifs basiques hélice-boucle-hélice et à glissière à leucines (métabolisme)</term>
<term>Gouttelettes lipidiques (composition chimique)</term>
<term>Gouttelettes lipidiques (effets des médicaments et des substances chimiques)</term>
<term>Gouttelettes lipidiques (métabolisme)</term>
<term>Métabolisme lipidique (effets des médicaments et des substances chimiques)</term>
<term>Phosphatidylinositol 3-kinases (génétique)</term>
<term>Phosphatidylinositol 3-kinases (métabolisme)</term>
<term>Pression osmotique (MeSH)</term>
<term>Protein Phosphatase 2 (génétique)</term>
<term>Protein Phosphatase 2 (métabolisme)</term>
<term>Protéines adaptatrices de la transduction du signal (génétique)</term>
<term>Protéines adaptatrices de la transduction du signal (métabolisme)</term>
<term>Protéines de Saccharomyces cerevisiae (antagonistes et inhibiteurs)</term>
<term>Protéines de Saccharomyces cerevisiae (génétique)</term>
<term>Protéines de Saccharomyces cerevisiae (métabolisme)</term>
<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Saccharomyces cerevisiae (effets des médicaments et des substances chimiques)</term>
<term>Saccharomyces cerevisiae (génétique)</term>
<term>Saccharomyces cerevisiae (métabolisme)</term>
<term>Sirolimus (pharmacologie)</term>
<term>Sorbitol (pharmacologie)</term>
<term>Transduction du signal (MeSH)</term>
<term>Triglycéride (biosynthèse)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Saccharomyces cerevisiae Proteins</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Triglycerides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>GATA Transcription Factors</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Adaptor Proteins, Signal Transducing</term>
<term>Basic Helix-Loop-Helix Leucine Zipper Transcription Factors</term>
<term>GATA Transcription Factors</term>
<term>Protein Phosphatase 2</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adaptor Proteins, Signal Transducing</term>
<term>Basic Helix-Loop-Helix Leucine Zipper Transcription Factors</term>
<term>Cholesterol Esters</term>
<term>Protein Phosphatase 2</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Transcription Factors</term>
</keywords>
<keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Facteurs de transcription</term>
<term>Protéines de Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Triglycéride</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Lipid Droplets</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Gouttelettes lipidiques</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lipid Droplets</term>
<term>Lipid Metabolism</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Facteurs de transcription</term>
<term>Facteurs de transcription GATA</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Gouttelettes lipidiques</term>
<term>Métabolisme lipidique</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phosphatidylinositol 3-Kinases</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de transcription</term>
<term>Facteurs de transcription GATA</term>
<term>Facteurs de transcription à motifs basiques hélice-boucle-hélice et à glissière à leucines</term>
<term>Phosphatidylinositol 3-kinases</term>
<term>Protein Phosphatase 2</term>
<term>Protéines adaptatrices de la transduction du signal</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lipid Droplets</term>
<term>Phosphatidylinositol 3-Kinases</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cholestérol ester</term>
<term>Facteurs de transcription</term>
<term>Facteurs de transcription à motifs basiques hélice-boucle-hélice et à glissière à leucines</term>
<term>Gouttelettes lipidiques</term>
<term>Phosphatidylinositol 3-kinases</term>
<term>Protein Phosphatase 2</term>
<term>Protéines adaptatrices de la transduction du signal</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Sirolimus</term>
<term>Sorbitol</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Sirolimus</term>
<term>Sorbitol</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term>
<term>Osmotic Pressure</term>
<term>Phosphoinositide-3 Kinase Inhibitors</term>
<term>Signal Transduction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Pression osmotique</term>
<term>Régulation de l'expression des gènes fongiques</term>
<term>Transduction du signal</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Lipid droplets (LDs) are intracellular structures that regulate neutral lipid homeostasis. In mammals, LD synthesis is inhibited by rapamycin, a known inhibitor of the mTORC1 pathway. In Saccharomyces cerevisiae, LD dynamics are modulated by the growth phase; however, the regulatory pathways involved are unknown. Therefore, we decided to study the role of the TORC1 pathway on LD metabolism in S. cerevisiae. Interestingly, rapamycin treatment resulted in a fast LD replenishment and growth inhibition. The discovery that osmotic stress (1 M sorbitol) also induced LD synthesis but not growth inhibition suggested that the induction of LDs in yeast is not a secondary response to reduced growth. The induction of LDs by rapamycin was due to increased triacylglycerol but not sterol ester synthesis. Induction was dependent on the TOR downstream effectors, the PP2A-related phosphatase Sit4p and the regulatory protein Tap42p. The TORC1-controlled transcriptional activators Gln3p, Gat1p, Rtg1p, and Rtg3p, but not Msn2p and Msn4p, were required for full induction of LDs by rapamycin. Furthermore, we show that the deletion of Gln3p and Gat1p transcription factors, which are activated in response to nitrogen availability, led to abnormal LD dynamics. These results reveal that the TORC1 pathway is involved in neutral lipid homeostasis in yeast. </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25512609</PMID>
<DateCompleted><Year>2015</Year>
<Month>04</Month>
<Day>13</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-5549</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>35</Volume>
<Issue>4</Issue>
<PubDate><Year>2015</Year>
<Month>Feb</Month>
</PubDate>
</JournalIssue>
<Title>Molecular and cellular biology</Title>
<ISOAbbreviation>Mol Cell Biol</ISOAbbreviation>
</Journal>
<ArticleTitle>TORC1 inhibition induces lipid droplet replenishment in yeast.</ArticleTitle>
<Pagination><MedlinePgn>737-46</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1128/MCB.01314-14</ELocationID>
<Abstract><AbstractText>Lipid droplets (LDs) are intracellular structures that regulate neutral lipid homeostasis. In mammals, LD synthesis is inhibited by rapamycin, a known inhibitor of the mTORC1 pathway. In Saccharomyces cerevisiae, LD dynamics are modulated by the growth phase; however, the regulatory pathways involved are unknown. Therefore, we decided to study the role of the TORC1 pathway on LD metabolism in S. cerevisiae. Interestingly, rapamycin treatment resulted in a fast LD replenishment and growth inhibition. The discovery that osmotic stress (1 M sorbitol) also induced LD synthesis but not growth inhibition suggested that the induction of LDs in yeast is not a secondary response to reduced growth. The induction of LDs by rapamycin was due to increased triacylglycerol but not sterol ester synthesis. Induction was dependent on the TOR downstream effectors, the PP2A-related phosphatase Sit4p and the regulatory protein Tap42p. The TORC1-controlled transcriptional activators Gln3p, Gat1p, Rtg1p, and Rtg3p, but not Msn2p and Msn4p, were required for full induction of LDs by rapamycin. Furthermore, we show that the deletion of Gln3p and Gat1p transcription factors, which are activated in response to nitrogen availability, led to abnormal LD dynamics. These results reveal that the TORC1 pathway is involved in neutral lipid homeostasis in yeast. </AbstractText>
<CopyrightInformation>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</CopyrightInformation>
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</ArticleIdList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3077-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12629214</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Biol. 2000 Nov 13;151(4):863-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11076970</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1991 Aug 23;253(5022):905-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1715094</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Histochem Cell Biol. 2008 Aug;130(2):263-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18546013</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1995 Nov 17;270(46):27531-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7499212</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Mol Cell Biol. 2007 Oct;8(10):774-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17712357</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2002 May 14;99(10):6784-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11997479</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Microbiol Biotechnol. 2011 May;90(4):1193-206</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21452033</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Syst Biol. 2011;7:545</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22068328</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lipids. 2010 Dec;45(12):1089-100</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21042876</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 1974 Jul 15;43(2):176-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4604647</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genetics. 2011 Dec;189(4):1177-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22174183</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Bacteriol. 1995 Dec;177(23):6910-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7592485</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 Apr 19;108(16):6349-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21464305</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2010;5(10):e13692</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21060891</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vis Exp. 2009;(30). pii: 1513. doi: 10.3791/1513</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19701157</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2008 Apr 25;30(2):214-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18439900</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect. 2010 Jan;60(1):65-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19837110</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2003 Nov 26;115(5):577-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14651849</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Cell Dev Biol. 2012;28:411-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22578141</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1999 Dec 9;402(6762):689-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10604478</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2006 Jan 6;281(1):491-500</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16267052</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2007 Mar;1771(3):255-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16950653</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Appl Microbiol. 2002;51:1-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12236054</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiol Mol Biol Rev. 2004 Jun;68(2):187-206</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15187181</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2002 Feb 22;277(8):6344-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11744721</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biotechnol. 1999 Aug;12(1):35-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10554772</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2007 Jun 8;26(5):663-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17560372</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Clin Nutr. 2011 Apr;93(4):891S-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21325438</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Dev. 1999 Dec 15;13(24):3271-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10617575</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2009 Jan 16;33(1):53-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19150427</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Biol. 2008 Nov 25;6(11):e292</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19067489</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2010 Mar 5;393(2):274-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20132792</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genetics. 2014 Oct;198(2):773-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25085507</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Biochem Cell Biol. 2006;38(9):1476-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16647875</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2008 Jan 25;283(4):2203-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18039669</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2006 Feb 10;124(3):471-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16469695</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2012 May 29;109(22):8652-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22589305</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 1990 Apr;10(4):1399-405</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2181273</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2002 Sep;10(3):457-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12408816</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS J. 2008 Nov;275(22):5552-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18959743</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Can J Biochem Physiol. 1959 Aug;37(8):911-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">13671378</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2009 Nov 6;284(45):30981-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19608739</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Prog Lipid Res. 2009 Nov;48(6):375-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19720081</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 1996 Mar;16(3):847-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8622686</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Dev. 1996 Aug 1;10(15):1904-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8756348</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2009 Dec 1;19(22):R1046-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19948145</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Jpn Acad Ser B Phys Biol Sci. 2009;85(7):217-28</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19644222</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1990 May 25;18(10):3091-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2190191</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Diabetes. 2010 Apr;59(4):775-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20068142</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Microbiol. 1969 Aug;18(2):245-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16349857</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Prostaglandins Leukot Essent Fatty Acids. 2010 Apr-Jun;82(4-6):243-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20206487</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Microbiol Rev. 2001 Jan;25(1):125-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11152943</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Prog Lipid Res. 2001 Sep;40(5):325-438</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11470496</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO Mol Med. 2013 Jul;5(7):905-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23740690</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2006 Aug 9;25(15):3546-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16874307</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eukaryot Cell. 2004 Oct;3(5):1261-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15470255</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2003 Jun;11(6):1467-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12820961</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Cell Biol. 2013;116:15-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24099285</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 1996 Jan;7(1):25-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8741837</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Yeast. 1994 Nov;10(11):1421-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7871881</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2010 Jun 4;285(23):17880-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20378536</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biotechnol. 2009 Apr 20;141(1-2):31-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19428728</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1994 Jul 29;269(30):19509-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7913470</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 1994 Jan;5(1):105-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8186460</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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   |clé=     pubmed:25512609
   |texte=   TORC1 inhibition induces lipid droplet replenishment in yeast.
}}

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	Serveur d'exploration sur la rapamycine et les champignons
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Serveur d'exploration sur la rapamycine et les champignons

TORC1 inhibition induces lipid droplet replenishment in yeast.

TORC1 inhibition induces lipid droplet replenishment in yeast.

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